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	Provedor de dados BJM (2) Autor Fukushima,Romualdo S. (1) Kunz,Daniel A. (1) Martin,Anandi (1) Palomino,Juan Carlos (1) Portaels,Françoise (1) Silva,Pedro Eduardo Almeida da (1) Weimer,Paul J. (1) von Groll,Andrea (1) Mais... Palavra-chave Resazurin (2) Anaerobiosis (1) Culture medium (1) Cysteine (1) Fitness (1) Growth kinetics (1) Light (1) Mycobacterium tuberculosis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2010 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies BJM von Groll,Andrea; Martin,Anandi; Portaels,Françoise; Silva,Pedro Eduardo Almeida da; Palomino,Juan Carlos. We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate. Tipo: Info:eu-repo/semantics/article Palavras-chave: Growth kinetics; Mycobacterium tuberculosis; Resazurin; Fitness. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200006 Use of photocatalytic reduction to hasten preparation of culture media for saccharolytic Clostridium species BJM Fukushima,Romualdo S.; Weimer,Paul J.; Kunz,Daniel A.. Cysteine is the preferred reducing agent used in the preparation of culture media for the growth of many strictly anaerobic microorganisms; however, redox potential reduction of cysteine is very slow, making it inconvenient if the medium is needed immediately or in large quantity. The time required to reduce culture medium containing resazurin (an indicator of reducing conditions) was dramatically shortened when the medium, after being injected with the reducing agent cysteine, was irradiated with incandescent light from a halogen lamp. Light intensity had an effect upon reduction time: tubes kept in the dark took more than 12 h to achieve the desired degree of anaerobiosis (measured spectrophotometrically by the bleaching of the indicator, resazurin)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Anaerobiosis; Culture medium; Cysteine; Light; Resazurin. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100006 Registros recuperados: 2 Primeira ... 1 ... Última

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